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The production of alcohol by cellulase enzymes using cellulose material as a substrate
- β-D Glucose can be produced from cellulose materials by cellulase enzymes. The cellulase enzyme breakdown β-1,4 glucosidic linkage and formed β-D Glucose. The conversion rate of cellulose to β-D glucose depends on the enzyme activity. In this study, two kinds of commercial cellulase namely Food Grade (FG) and Technical Grade (TG) enzymes were used for the saccharification of celluolosic materials such as rice straw, grass, water hyacinth, bagasse and corn cob and then studied on the determination of reducing sugar by Filter Paper Assay or Filter Paper Degrading (FPD) Method. In this study, the optimal parameters of enzyme concentration, weight of substrate, reaction time, temperature and pH of TG were found to be enzyme 0.6 ml (range from 0.2-1.0 ml), 0.4 g of substrates (range from 0.1-1.0 g), for 4 hours reaction time (range from 1-24 hr), at 50°C (range from 30-70°C) and pH 4 (range from 3.0-5.5) for rice straw, grass, water hyacinth, bagasse and corn cob, respectively. Similarly, enzyme 0.4 ml, 0.4 g of substrates, 4 hours reaction time, at 50°C and pH 4.5 were the optimal parameters of FG on rice straw, grass, water hyacinth, bagasse and corn cob, respectively. The effect of fermentation period of enzymes TG on the production of alcohol was studied using rice straw and water hyacinth and using rice straw and bagasse for FG, respectively. According to the results 48 hr fermentation period was the most suitable reaction time for both TG and FG. Lab scale alcohol fermentation production was conducted using rice straw with optimal parameters. The result showed that 48 hours fermentation period, temperature at 30°C and pH-5 and 5.5 by TG and FG, respectively, gave the best alcohol concentration.
Thi Thi Than Myint