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Culture of Musa chiliacarpa Back in Murashige and Skoog Liquid Medium For Shoot Proliferation and Cell Types
- The selected callus from modified MS solid medium were transferred into MS liquid medium for determination of shoot proliferation and cell types of Musa chiliocarpa Back. The MS liquid medium for callus proliferation was composed a combination of plant growth regulators such as auxin and cytokinin: 0.25 mg/l IAA + lmg/l BAP, 0.25 mg/l IAA + 2mgtl BAP and 0.25 mg/l IAA + 3mg/l BAP. Similarly, 0.5 mg/l, IAA and 0.75 mg/l IAA, each was mixed with I, 2 and 3 mg/l BAP solution. The suspension MS medium supplemented with different concentrations of IAA and BAP such as I mg/l IAA + 1, 3 and 5 mg/l BAP; 2mgtl IAA + 1, 3 and 5 mg/l BAP and 3 mg/l IAA + 1, 3 and 5 mg/l BAP was used to determine the cell types of M. chiliocarpa Back. Most of cells were isodiametric and single but some were clusters, round, oval, rod, filamentous and elongated shaped.
Cho Cho Nyunt
San San Aye
- Universities Research Journal (URJ)