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An-Antioxidant Organie Compound Isolated from the Stem of Hypericum calycinum L. (Pyin-nyar-lin-kar)
- In the present study, stem of Hypericum calycinum L. (Pyin-nyar-lin-kar in Myanmar), which is mainly used in the treatment of malaria in Myanmar Traditional Medicinal System, was selected for chemical investigation. The preliminary phytochemical screening of the stem of this plant was performed. Moreover, ant bacterial tests of the crude extracts of this plant sample were done by agar well diffusion method in various solvents on six selected organisms. The antioxidant activities of three crude extracts of Hypericum ca/ycinum L. were determined by using 1-1-diphenyl-2-picryl hydrazyl (DPPH) assay. In addition, the pure colourless crystal was isolated from the ethyl acetate extract of the stem of Hypericum calycinum L. by using loin Layer and Column Chromatographic methods. The phytochemical test of this compound gave positive result for flavonoid group only. The molecular formula of this compound could be assigned as C25H28O6 based upon some advanced spectroscopic methods. The complete structure of this new compound was elucidated on the basis of 10 and 2D-NMR analysis as well as mass spectrometry (EI and HR-MS). The scientific name of this elucidated compound is named as 5-hydroxy-2S-[2, 4, 6-trihydroxy)-3-(2isoprophenyl- 5-methyl-hex-4-enyl) phenyl]-chroman-4 one. In order to know the antioxidant activity of this compound, Oxygen Radical Absorbance Capacity (ORAC) assay was performed. Moreover, cytotoxic activity of this compound was done by Colorimetric MTT (tetrazolium) assay on HeLa human cervix carcinoma cell and Coca-2 human epithelial colorectal adenocarcinoma cell.
Thant Thant Htwe
Myint Myint Sein
- Universities Research Journal (URJ)