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Investigation of the Antioxidant Activity of Cydonia cathayensis Hemsl. (Chinsaw-ga) Fruit
- This paper presents the determination of the antioxidant activity of chinsaw-ga fruit (Cydonia cathayensis Hemsl.). Free radicals can cause oxidative damage to biomolecules of living systems. Antioxidants which scavenge free radicals can prevent the oxidation of biomolecules. Firstly, rapid screening of antioxidant activity of 95% ethanol, ethyl acetate and watery extracts of Cydonia cathayensis fruit were carried out by dot-blot and DPPH staining method. After achieving the results which shewed radical scavenging activity, antioxidant activity of these fruit extracts were determined by DPPH assay using UV-Vis spectrophotometric method. In this assay, ascorbic acid (vitamin C) was used as a standard. Antioxidant activity is expressed as 50% inhibition concentration (IC50) values. The lower IC50value indicates the greater antioxidant activity. IC50 values for ethyl acetate, ethanol and watery extracts of Cydonia cathayensis fruit were found to be 32.51, 20.51 and 34.12 µg/mL respectively. Ethanol extract showed the most potent antioxidant activity.
San San Oo
- Universities Research Journal (URJ)