Isolation and Structural Elucidation of an Unknown Biologically Active Compound from Myanmar Traditional Indigenous Medicinal Plant Clerodendrum serratum SPRENG (Yin-bya-net

Abstract

In this Ph.D research work, one well-known Myanmar Indigenous Medicinal Plant, namely Clerodendrum serratum Spreng. (Yin-bye-net) was chosen (or preliminary phytochemical screening and antibacterial activities. In accordance with phyto test the crude extract of root of this plant responds some chemical constituents, such as, alkaloid, phenolic, lipophilic, polyphenol and glycoside, respectively. Moreover, antibacterial activities of this crude extract in various solvent systems were done by agar well diffusion method on three selected organisms (Bacillus Subtilis, Staphlococcus aureus and Pseudomonous aeruginosa). Among them ethyl acetate extract of this plant gave rise to medium activities on all tested organisms. Furthermore a pure white amorphous unknown alkaloid compound (DAM-1) was isolated from ethyl acetate extract of root of Yin-bya-net by some modem separation methods, such as Thin Layer and Column Chromatographic methods. The yield percent of this unknown alkaloid could be calculated as (0.85%) based upon the ethyl acetate crude extract. The molecular formula of this unknown potent alkaloid could be assigned as (C13H21O4N3) by some sophisticated spectroscopic methods, such as, FT-IR ,1H NMR(600 MHz), 13C NMR (I50MHz) , DEPT, HSQC and EI-Mass spectrums.